Validation Data Gallery
Tested Applications
| Positive WB detected in | RT-4 cells, HepG2 cells, Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26626-1-AP targets RGL3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24329 Product name: Recombinant human RGL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-251 aa of BC101756 Sequence: TEDGAVYSVSLRRQRSQRRSPAEGPGGSQAPSPIANTFLHYRTSKVRVLRAARLERLVGELVFGDREQDPSFMPAFLATYRTFVPTACLLGFLLPPMPPPPPPGVEIKKTAVQDLSFNKNLRAVVSVLGSWLQDHPQDFRDPPVHSDLGSVRTFLGWAAPGSAEAQKAEKLLEDFLEEAEREQEEEPPQVWTGPPRVAQTSDPDSSEACAEEEEGLMPQGPQLLDFSVDEV 相同性解析による交差性が予測される生物種 |
| Full Name | ral guanine nucleotide dissociation stimulator-like 3 |
| Calculated molecular weight | 710 aa, 78 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | BC101756 |
| Gene Symbol | RGL3 |
| Gene ID (NCBI) | 57139 |
| RRID | AB_3669556 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3MIN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RGL3 is a guanine nucleotide exchange factor. RGL3 mRNA is commonly detectable in several visceral organs (e.g. kidney, heart, liver, and lung) in the mouse and human. The RGL3 protein mainly localizes in the cytoplasm when expressed in fibroblasts. Yeast two-hybrid assay indicated that RGL3 could interact with Rap1, Rap2, H-Ras, N-Ras, and R-Ras but failed to interact efficiently with Ral and Rho (PMID: 17382517).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RGL3 antibody 26626-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |