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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFX2 Polyclonal antibody (12622-1-AP)

    WB analysis using 12622-1-AP (same clone as 12622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12622-1-AP (RFX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of THP-1 cells using RFX2 Polyclonal antibody (12622-1-AP)

    IP experiment of THP-1 using 12622-1-AP (same clone as 12622-1-PBS)

    IP result of anti-RFX2 (IP:12622-1-AP, 4ug; Detection:12622-1-AP 1:1000) with THP-1 cells lysate 1920 ug. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

RFX2 Polyclonal antibody, PBS Only

RFX2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12622-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

DNA binding protein RFX2, DNA-binding protein RFX2, Regulatory factor X 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12622-1-PBS targets RFX2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3310

Product name: Recombinant human RFX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 424-723 aa of BC028579

Sequence: VLPKDKLISLCQCDPILRWMRSCDHILYQALVEILIPDVLRPVPSTLTQAIRNFAKSLEGWLTNAMSDFPQQVIQTKVGVVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEESVVQRLEQDFKLTLQQQSSLDQWASWLDSVVTQVLKQHAGSPSFPKAAQQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAEATGETPIAVMGEFNDLASLSLTLLDKDDMGDEQRGSEAGPDARSLGEPLVKRERSDPNHSLQGI

相同性解析による交差性が予測される生物種
Full Name regulatory factor X, 2 (influences HLA class II expression)
Calculated molecular weight 723 aa, 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC028579
Gene Symbol RFX2
Gene ID (NCBI) 5990
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48378
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RFX2 is a key regulator of ciliogenesis in vertebrates, and knockdown of Rfx2 causes defects in neural tube closure and in left-right axis patterning (PMID: 26248850). RFX2 expression is greatly enhanced in adult testis and that RFX2 is equally prominent in highly enriched populations of late pachytene spermatocytes and round spermatids(PMID: 22227339). RFX2 is also required for the development of left-right asymmetry, and motile cilia on RFX2-expressing cells are sufficient to establish adequate fluid flow across the COA to direct normal LR development in mouse (PMID: 22233545).

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