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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFX2 Polyclonal antibody (12622-1-AP)

    WB analysis using 12622-1-AP (same clone as 12622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12622-1-AP (RFX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of THP-1 cells using RFX2 Polyclonal antibody (12622-1-AP)

    IP experiment of THP-1 using 12622-1-AP (same clone as 12622-1-PBS)

    IP result of anti-RFX2 (IP:12622-1-AP, 4ug; Detection:12622-1-AP 1:1000) with THP-1 cells lysate 1920 ug. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RFX2 Polyclonal antibody (12622-1-AP)

    IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

RFX2 Polyclonal antibody, PBS Only

RFX2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12622-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

DNA binding protein RFX2, DNA-binding protein RFX2, Regulatory factor X 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12622-1-PBS targets RFX2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3310

Product name: Recombinant human RFX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 424-723 aa of BC028579

Sequence: VLPKDKLISLCQCDPILRWMRSCDHILYQALVEILIPDVLRPVPSTLTQAIRNFAKSLEGWLTNAMSDFPQQVIQTKVGVVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEESVVQRLEQDFKLTLQQQSSLDQWASWLDSVVTQVLKQHAGSPSFPKAAQQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAEATGETPIAVMGEFNDLASLSLTLLDKDDMGDEQRGSEAGPDARSLGEPLVKRERSDPNHSLQGI

相同性解析による交差性が予測される生物種
Full Name regulatory factor X, 2 (influences HLA class II expression)
Calculated molecular weight 723 aa, 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC028579
Gene Symbol RFX2
Gene ID (NCBI) 5990
RRIDAB_3741832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48378
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RFX2 is a key regulator of ciliogenesis in vertebrates, and knockdown of Rfx2 causes defects in neural tube closure and in left-right axis patterning (PMID: 26248850). RFX2 expression is greatly enhanced in adult testis and that RFX2 is equally prominent in highly enriched populations of late pachytene spermatocytes and round spermatids(PMID: 22227339). RFX2 is also required for the development of left-right asymmetry, and motile cilia on RFX2-expressing cells are sufficient to establish adequate fluid flow across the COA to direct normal LR development in mouse (PMID: 22233545).

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