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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using RFWD3 Polyclonal antibody (19893-1-AP)

    WB analysis of mouse lung using 19893-1-AP (same clone as 19893-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RFWD3 Polyclonal antibody (19893-1-AP)

    IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RFWD3 Polyclonal antibody (19893-1-AP)

    IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RFWD3 Polyclonal antibody (19893-1-AP)

    IHC staining of human colon using 19893-1-AP (same clone as 19893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RFWD3 Polyclonal antibody (19893-1-AP)

    IHC staining of human colon using 19893-1-AP (same clone as 19893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RFWD3 Polyclonal antibody (19893-1-AP)

    IF Staining of HEK-293 using 19893-1-AP (same clone as 19893-1-PBS)

    Immunofluorescent analysis of 293 cells, using RFWD3 antibody 19893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RFWD3 Polyclonal antibody, PBS Only

RFWD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19893-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RFWD3, EC:2.3.2.27, RING finger and WD repeat domain-containing protein 3, RING finger protein 201, RNF201

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19893-1-PBS targets RFWD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13728

Product name: Recombinant human RFWD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 427-774 aa of BC002574

Sequence: SQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

相同性解析による交差性が予測される生物種
Full Name ring finger and WD repeat domain 3
Calculated molecular weight 774 aa, 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC002574
Gene Symbol RFWD3
Gene ID (NCBI) 55159
RRIDAB_10639521
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PCD5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

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