Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
24289-1-AP targets RFTN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RFTN1 fusion protein Ag17264 相同性解析による交差性が予測される生物種 |
| Full Name | raftlin, lipid raft linker 1 |
| Calculated molecular weight | 578 aa, 63 kDa |
| Observed molecular weight | 63-70 kDa |
| GenBank accession number | BC100667 |
| Gene Symbol | RFTN1 |
| Gene ID (NCBI) | 23180 |
| RRID | AB_2879474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Raftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RFTN1 antibody 24289-1-AP | Download protocol |
| IHC protocol for RFTN1 antibody 24289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Angiogenesis Raftlin is recruited by neuropilin-1 to the activated VEGFR2 complex to control proangiogenic signaling. | ||
Pathol Res Pract RFTN1 facilitates gastric cancer progression by modulating AKT/p38 signaling pathways.
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