Validation Data Gallery
Product Information
Alpaca anti-RFP VHH, purified recombinant binding protein
| Applications | Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction |
| Specificity/Target | mCherry, mRFP, mRFPRuby, TagRFP, mKate2, mPlum, mApple, DsRed, tdTomato For the complete list, please click here: Fluorescent protein specificity table |
| Conjugate | Unconjugated |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 5 nM |
| Molecular Weight | 14.9 kDa |
| RRID | AB_2631365 |
| Storage Buffer | 25mM TAPS pH 8.5, 137 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze. |
| Size | 250 µg |
| Background | Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This Nanobody is an alpaca recombinant VHH raised against RFP, reactive to a variety of RFP derivates, including mCherry, mRFP, mRFPRuby, TagRFP, mKate2, mPlum, mApple, DsRed, and tdTomato. |
Documentation
| SDS |
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| rt_SDS_RFP VHH, recombinant binding protein (EN) |
| Datasheet |
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| RFP VHH recombinant binding protein datasheet (PDF) |
Publications
| Application | Title |
|---|---|
Nat Methods Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. | |
Plant J BPC transcription factors and a Polycomb Group protein confine the expression of the ovule identity gene SEEDSTICK in Arabidopsis. | |
ACS Chem Biol A simple method for GFP- and RFP-based dual color single-molecule localization microscopy. | |
Cell Chem Biol The cyclimids: Degron-inspired cereblon binders for targeted protein degradation | |
J Vasc Res Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis |