Validation Data Gallery
![HeLa cells transiently transfected with
MannosidaseII-tdTomato (Golgi).
Primary antibody: [5F8] 1:1,000
Secondary antibody: goat anti-rat Alexa Fluor® 647
Fixation: 3.7 % PFA
Scale bar: 10 µm](https://www.ptglab.com/Products/Pictures/Result-5f8-2.png "HeLa cells transiently transfected with
MannosidaseII-tdTomato (Golgi).
Primary antibody: [5F8] 1:1,000
Secondary antibody: goat anti-rat Alexa Fluor® 647
Fixation: 3.7 % PFA
Scale bar: 10 µm")
Product Information
Rat monoclonal antibody [5F8] to Red Fluorescent Proteins
| Description | Rat monoclonal antibody [5F8] to Red Fluorescent Proteins |
| Applications | IF, ELISA |
| Specificity/Target | Tested on DsRed, mRFP, mCherry, mPlum, mRFPruby, mScarlet, tdTomato, mKate2, mOrange. |
| Recommended Dilution | IF: recommended starting dilution 1:1,000 A titration from 1:200 up to 1:2,000 is recommended. |
| Host/IsoType | Rat/IgG2a |
| Immunogen | UniProtKB: Q9U6Y8 |
| Type | Primary Antibody |
| Conjugate | Unconjugated |
| Clonality | Monoclonal |
| Clone No. | 5F8 |
| RRID | AB_2336064 |
| Purity | Protein A or G purified |
| Form | Buffered aqueous solution |
| Storage Buffer | PBS, 50% glycerol, preservative: 0.09% sodium azide. |
| Storage Condition | Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment. |
| Size | 20 μL; 150 μL |
Publications
| Application | Title |
|---|---|