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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using RFFL Polyclonal antibody (12687-1-AP)

    WB analysis of mouse testis using 12687-1-AP (same clone as 12687-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using RFFL Polyclonal antibody (12687-1-AP)

    WB analysis of mouse thymus using 12687-1-AP (same clone as 12687-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using RFFL Polyclonal antibody (12687-1-AP)

    WB analysis of mouse testis using 12687-1-AP (same clone as 12687-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12687-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using RFFL Polyclonal antibody (12687-1-AP)

    IP experiment of mouse testis using 12687-1-AP (same clone as 12687-1-PBS)

    IP result of anti-RFFL (IP:12687-1-AP, 4ug; Detection:12687-1-AP 1:300) with mouse testis tissue lysate 3520 ug. This data was developed using the same antibody clone with 12687-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RFFL Polyclonal antibody, PBS Only

RFFL Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12687-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12687-1-PBS targets RFFL in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3380

Product name: Recombinant human RFFL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-327 aa of BC028424

Sequence: MWATCCNWFCLDGQPEEVPPPQGARMQAYSNPGYSSFPSPTGLEPSCKSCGAHFANTARKQTCLDCKKNFCMTCSSQVGNGPRLCLLCQRFRATAFQREELMKMKVKDLRDYLSLHDISTEMCREKGELVLLVLGQQPVISQEDRTRASTLSPDFPEQQAFLTQPHSSMVPPTSPNLPSSSAQATSVPPAQVQENQQSIDSEDSFVPGRRASLSDLTDLEDIEGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLGGAVPSGLEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMNECPICRQYVIRAVHVFRS

相同性解析による交差性が予測される生物種
Full Name ring finger and FYVE-like domain containing 1
Calculated molecular weight 327 aa, 37 kDa
Observed molecular weight 37-41 kDa
GenBank accession numberBC028424
Gene Symbol RFFL
Gene ID (NCBI) 117584
RRIDAB_10644289
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WZ73
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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