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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFC3 Monoclonal antibody (68290-1-Ig)

    WB analysis using 68290-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using RFC3 Monoclonal antibody (68290-1-Ig)

    WB analysis of NIH/3T3 using 68290-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using RFC3 Monoclonal antibody (68290-1-Ig)

    WB analysis of HSC-T6 using 68290-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RFC3 Monoclonal antibody

RFC3 Monoclonal Antibody for WB, ELISA
Cat No. 68290-1-Ig
Clone No.2G3A8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Synonyms

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68290-1-Ig targets RFC3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28365

Product name: Recombinant human RFC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-356 aa of BC000149

Sequence: MSLWVDKYRPCSLGRLDYHKEQAAQLRNLVQCGDFPHLLVYGPSGAGKKTRIMCILRELYGVGVEKLRIEHQTITTPSKKKIEISTIASNYHLEVNPSDAGNSDRVVIQEMLKTVAQSQQLETNSQRDFKVVLLTEVDKLTKDAQHALRRTMEKYMSTCRLILCCNSTSKVIPPIRSRCLAVRVPAPSIEDICHVLSTVCKKEGLNLPSQLAHRLAEKSCRNLRKALLMCEACRVQQYPFTADQEIPETDWEVYLRETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVAKFMALYKKFMEDGLEGMMF

相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 3, 38kDa
Calculated molecular weight 40 aa, 6 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000149
Gene Symbol RFC3
Gene ID (NCBI) 5983
RRIDAB_2935370
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP40938
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.

Protocols

Product Specific Protocols
WB protocol for RFC3 antibody 68290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols