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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFC1 Recombinant antibody (85148-4-RR)

    WB analysis using 85148-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85148-4-RR (RFC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using RFC1 Recombinant antibody (85148-4-RR)

    IHC staining of human colon using 85148-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85148-4-RR (RFC1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RFC1 Recombinant antibody (85148-4-RR)

    IF Staining of HeLa using 85148-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC1 antibody (85148-4-RR, Clone: 242818H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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RFC1 Recombinant antibody

RFC1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85148-4-RR
Clone No.242818H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293T cells, A431 cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85148-4-RR targets RFC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RFC1 fusion protein Ag13585 相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 1, 145kDa
Calculated molecular weight 128 kDa
Observed molecular weight140 kDa
GenBank accession numberBC051751
Gene Symbol RFC1
Gene ID (NCBI) 5981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35251
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).

Protocols

Product Specific Protocols
WB protocol for RFC1 antibody 85148-4-RRDownload protocol
IHC protocol for RFC1 antibody 85148-4-RRDownload protocol
IF protocol for RFC1 antibody 85148-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols