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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REXO4 Recombinant antibody (86528-1-RR)

    WB analysis using 86528-1-RR (same clone as 86528-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86528-1-RR (REXO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86528-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using REXO4 Recombinant antibody (86528-1-RR)

    IF Staining of HepG2 using 86528-1-RR (same clone as 86528-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using REXO4 antibody (86528-1-RR, Clone: 251440H2 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86528-1-PBS in a different storage buffer formulation.

REXO4 Recombinant monoclonal antibody, PBS Only

REXO4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86528-1-PBS
Clone No.251440H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:3.1.-.-, hPMC2, PMC2, REX 4, REX4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86528-1-PBS targets REXO4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13473

Product name: Recombinant human REXO4,REX4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 71-422 aa of BC009274

Sequence: WKALQEWLLKQKSQAPEKPLVISQMGSKKKPKIIQQNKKETSPQVKGEEMPAGKDQEASRGSVPSGSKMDRRAPVPRTKASGTEHNKKGTKERTNGDIVPERGDIEHKKRKAKEAAPAPPTEEDIWFDDVDPADIEAAIGPEAAKIARKQLGQSEGSVSLSLVKEQAFGGLTRALALDCEMVGVGPKGEESMAARVSIVNQYGKCVYDKYVKPTEPVTDYRTAVSGIRPENLKQGEELEVVQKEVAEMLKGRILVGHALHNDLKVLFLDHPKKKIRDTQKYKPFKSQVKSGRPSLRLLSEKILGLQVQQAEHCSIQDAQAAMRLYVMVKKEWESMARDRRPLLTAPDHCSDDA

相同性解析による交差性が予測される生物種
Full Name REX4, RNA exonuclease 4 homolog (S. cerevisiae)
Calculated molecular weight 422 aa, 47 kDa
Observed molecular weight50 kDa
GenBank accession numberBC009274
Gene Symbol REXO4
Gene ID (NCBI) 57109
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9GZR2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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