Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
18170-1-AP targets Resistin in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12822 Product name: Recombinant human RETN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-108 aa of BC101560 Sequence: EAINERIQEVAGSLIFRAISSIGLECQSVTSRGDLATCPRGFAVTGCTCGSACGSWDVRAETTCHCQCAGMDWTGARCCRVQP 相同性解析による交差性が予測される生物種 |
| Full Name | resistin |
| Calculated molecular weight | 108 aa, 11 kDa |
| GenBank accession number | BC101560 |
| Gene Symbol | RETN |
| Gene ID (NCBI) | 56729 |
| RRID | AB_2918058 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD89 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Resistin is a member of the resistin-like molecule family (RELMs) that includes three additional proteins, RELM-α, RELM-β, RELM-y . Resistin is almost exclusively expressed in white adipocytes in mice, whereas macrophages are the primary source in humans . In a variety of pathophysiologic states, particularly in type 2 diabetes mellitus and in chronic kidney disease, the serum concentration of resistin is increased. (PMID: 32822824, PMID: 19708984)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Resistin antibody 18170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Med (Lausanne) Resistin Expression Is Associated With Interstitial Lung Disease in Dermatomyositis. | ||
Cell Rep Med Clinical-proteomic classification and precision treatment strategy of chordoma | ||
PLoS Genet Single-cell RNA profiling reveals classification and characteristics of mononuclear phagocytes in colorectal cancer |