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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REST Recombinant antibody (84846-4-RR)

    WB analysis using 84846-4-RR (same clone as 84846-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84846-4-RR (REST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using REST Recombinant antibody (84846-4-RR)

    IF Staining of HeLa using 84846-4-RR (same clone as 84846-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REST antibody (84846-4-RR, Clone: 241789D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84846-4-RR

    Affinity and Kinetic Characterization of 84846-4-RR

    Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of REST Antibody (84846-4-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCRThe ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of YY1 Antibody (82712-3-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR

  • ChIP experiment of HeLa cells using REST Recombinant antibody (84846-4-RR)

    ChIP experiment of HeLa using 84846-4-RR (same clone as 84846-4-PBS)

    Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of REST Antibody (84846-4-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

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REST Recombinant antibody, PBS Only

REST Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84846-4-PBS
Clone No.241789D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

XBR, X2 box repressor, NRSF, 241789D3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84846-4-PBS targets REST in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen REST fusion protein Ag17707 相同性解析による交差性が予測される生物種
Full Name RE1-silencing transcription factor
Calculated molecular weight 1097 aa, 122 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC132859
Gene Symbol REST
Gene ID (NCBI) 5978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13127
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as a transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected at 200 kDa by western blot in our result, which is similar to the article (PMID: 16764822).