Validation Data Gallery
Tested Applications
| Positive WB detected in | T-47D cells, LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30670-1-AP targets REPS2 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33764 Product name: Recombinant human REPS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 390-620 aa of NM_004726 Sequence: LPANQQPRDLNRMEKTSVKDMADLPVPNQDVTSDDKQALKSTINEALPKDVSEDPATPKDSNSLKARPRSRSYSSTSIEEAMKRGEDPPTPPPRPQKTHSRASSLDLNKVFQPSVPATKSGLLPPPPALPPRPCPSQSEQVSEAELLPQLSRAPSQAAESSPAKKDVLYSQPPSKPIRRKFRPENQATENQEPSTAASGPASAATMKPHPTVQKQSSKQKKAIQTAIRKNK 相同性解析による交差性が予測される生物種 |
| Full Name | RALBP1 associated Eps domain containing 2 |
| Calculated molecular weight | 72KD |
| Observed molecular weight | 58 kDa, 78 kDa |
| GenBank accession number | NM_004726 |
| Gene Symbol | REPS2 |
| Gene ID (NCBI) | 9185 |
| RRID | AB_3086383 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NFH8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
REPS2 is part of a protein complex that regulates the endocytosis of growth factor receptors. The encoded protein directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. There are 4 isoforms of the protein with two short (58 kDa) isoforms comprising 521 or 522 amino acid residues and two long (78 kDa) ones of 659 or 660 residues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REPS2 antibody 30670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |