Validation Data Gallery
Tested Applications
| Positive WB detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31209-1-AP targets RENBP in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35056 Product name: Recombinant human RENBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-417 aa of BC015558 Sequence: MEKERETLQAWKERVGQELDRVVAFWMEHSHDQEHGGFFTCLGREGRVYDDLKYVWLQGRQVWMYCRLYRTFERFRHAQLLDAAKAGGEFLLRYARVAPPGKKCAFVLTRDGRPVKVQRTIFSECFYTMAMNELWRATGEVRYQTEAVEMMDQIVHWVQEDASGLGRPQLQGAPAAEPMAVPMMLLNLVEQLGEADEELAGKYAELGDWCARRILQHVQRDGQAVPENVSEGGKELPGCLGRQQNPGHTLEAGWFLLRHCIRKGDPELRAHVIDKFLLLPFHSGWDPDHGGLFYFQDADNFCPTQLEWAMKLWWPHSEAMIAFLMGYSDSGDPVLLRLFYQVAEYTFRQFRDPEYGEWFGYLSREGKVALSIKGGPFKGCFHVPRCLAMCEEMLGALLSRPAPAPSPAPTPACRGAE 相同性解析による交差性が予測される生物種 |
| Full Name | renin binding protein |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 29 kDa, 45-49 kDa |
| GenBank accession number | BC015558 |
| Gene Symbol | RENBP |
| Gene ID (NCBI) | 5973 |
| RRID | AB_3669902 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P51606 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Renin binding protein (RENBP) was originally identified as a protein in the kidney that was capable of binding renin in renal homogenates giving rise to a complex designated high molecular weight renin (PMID: 12866502). Studies have shown that human RnBP is the enzyme N-acetyl-D-glucosamine (GlcNAc) 2-epimerase (PMID: 12866502). RENBP has 3 isoforms produced by alternative splicing and alternative initiation with molecular weights of 29, 48, and 49 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RENBP antibody 31209-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |