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  • Featured Product
  • KD/KO Validated

REEP6 Polyclonal antibody

REEP6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12088-1-AP

Synonyms

C19orf32, DP1L1, receptor accessory protein 6, REEP6, TB2L1

Validation Data Gallery

Applications:
  • WB analysis of BxPC-3 using 12088-1-AP

    WB analysis of BxPC-3 using 12088-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12088-1-AP (REEP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12088-1-AP

    IHC staining of human pancreas cancer using 12088-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 12088-1-AP

    IHC staining of human pancreas cancer using 12088-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse olfactory epithelium tissue using 12088-1-AP

    IF Staining of mouse olfactory epithelium tissue using 12088-1-AP

    Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of REEP6 antibody (12088-1-AP, 1:100). Excellent staining of Sus cells on the apical layer as well as their basal projections. By Dr. Brian Lin (Schwob Lab).

Tested Applications

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse olfactory epithelium tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

12088-1-AP targets REEP6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP6 fusion protein Ag2723 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 6
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC008201
Gene symbol REEP6
Gene ID (NCBI) 92840
RRIDAB_2178460
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for REEP6 antibody 12088-1-APDownload protocol
IHC protocol for REEP6 antibody 12088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
View Article
ratWB

J Clin Invest

Nasal neuron PET imaging quantifies neuron generation and degeneration.

Authors - Genevieve C Van de Bittner
View Article
humanWB,IF

Am J Hum Genet

Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa.

Authors - Gavin Arno
View Article
mouseIF

Hum Mol Genet

REEP6 deficiency leads to retinal degeneration through disruption of ER homeostasis and protein trafficking.

Authors - Smriti A Agrawal
View Article