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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REEP5 Monoclonal antibody (68119-1-Ig)

    WB analysis using 68119-1-Ig (same clone as 68119-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using REEP5 Monoclonal antibody (68119-1-Ig)

    WB analysis of HeLa using 68119-1-Ig (same clone as 68119-1-PBS)

    WB result of REEP5 antibody (68119-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    WB analysis of pig heart using 68119-1-Ig (same clone as 68119-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    IHC staining of human colon cancer using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    IHC staining of human liver using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    IHC staining of human liver using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using REEP5 Monoclonal antibody (68119-1-Ig)

    IF Staining of A549 using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (68119-1-Ig, Clone: 1H8D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50780-1

    Cytometric bead array standard curve of MP50780-1

    Cytometric bead array standard curve of MP50780-1, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-2-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50780-2

    Cytometric bead array standard curve of MP50780-2

    Cytometric bead array standard curve of MP50780-2, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-3-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

REEP5 Monoclonal antibody, PBS Only (Capture)

REEP5 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68119-1-PBS
Clone No.1H8D8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1, C5orf18

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68119-1-PBS targets REEP5 as part of a matched antibody pair:

MP50780-1: 68119-1-PBS capture and 68119-2-PBS detection (validated in Cytometric bead array)

MP50780-2: 68119-1-PBS capture and 68119-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen REEP5 fusion protein Ag6229 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 5
Calculated molecular weight 21 kDa
Observed molecular weight18 kDa
GenBank accession numberBC065926
Gene Symbol REEP5
Gene ID (NCBI) 7905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00765
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).