Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, mouse testis tissue, U-251 cells, BxPC-3 cells, HaCaT cells, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86164-2-RR targets REEP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | REEP4 fusion protein Ag24569 相同性解析による交差性が予測される生物種 |
| Full Name | receptor accessory protein 4 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC013048 |
| Gene Symbol | REEP4 |
| Gene ID (NCBI) | 80346 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H6H4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
REEP4 (Receptor Expression-Enhancing Protein 4) is a member of the REEP family of proteins, a transmembrane protein that interacts with odorant receptor proteins and may enhance the odorant receptor responses to odorants. It is localized to the endoplasmic reticulum (ER) and is associated with the nuclear envelope (NE). REEP4 helps link the high-curvature ER to the ELYS-based nuclear pore complex (NPC) seed, thereby promoting NPC biogenesis during late mitosis. REEP4 expression is associated with the cell cycle, osteoclast differentiation, Fc gamma R-mediated phagocytosis, and Human T-cell leukemia virus 1 infection. (PMID: 36191880; 38947393)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP4 antibody 86164-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |