Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23140-1-AP targets REEP3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19400 Product name: Recombinant human REEP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 73-255 aa of BC068557 Sequence: VIWLLSPYTKGASLIYRKFLHPLLSSKEREIDDYIVQAKERGYETMVNFGRQGLNLAATAAVTAAVKSQGAITERLRSFSMHDLTTIQGDEPVGQRPYQPLPEAKKKSKPAPSESAGYGIPLKDGDEKTDEEAEGPYSDNEMLTHKGLRRSQSMKSVKTTKGRKEVRYGSLKYKVKKRPQVYF 相同性解析による交差性が予測される生物種 |
| Full Name | receptor accessory protein 3 |
| Calculated molecular weight | 255 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC068557 |
| Gene Symbol | REEP3 |
| Gene ID (NCBI) | 221035 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6NUK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
REEP3 (Receptor Expression-Enhancing Protein 3) is a member of the REEP family of proteins, a transmembrane protein that interacts with odorant receptor proteins and may enhance the odorant receptor responses to odorants. It primarily regulates the tubular morphology of the endoplasmic reticulum by promoting high membrane curvature through its reticular homology domains. During mitosis, REEP3 binds to microtubules, separating the endoplasmic reticulum from chromosomes to ensure proper nuclear membrane remodeling and cell division.(PMID: 23911198; 30995177)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP3 antibody 23140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |