Validation Data Gallery

Filter: AllWBIPIHCIF-PFC (Intra)

WB analysis using 15684-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15684-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye using 15684-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 15684-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15684-1-AP
IP result of anti-REEP2 (IP:15684-1-AP, 4ug; Detection:15684-1-AP 1:5000) with mouse brain tissue lysate 1680 ug.
IHC staining of mouse brain using 15684-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15684-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 15684-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15684-1-AP (REEP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of SH-SY5Y using 15684-1-AP
1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug REEP2 Polyclonal antibody (15684-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

REEP2 Polyclonal antibody

REEP2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 15684-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

C5orf19, Receptor expression-enhancing protein 2, SGC32445

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human heart tissue, mouse eye tissue, human skeletal muscle tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive FC (Intra) detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 12 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

15684-1-AP targets REEP2 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8186 Product name: Recombinant human REEP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 70-252 aa of BC006218 Sequence: IAFVIWLLSPYTKGSSVLYRKFVHPTLSNKEKEIDEYITQARDKSYETMMRVGKRGLNLAANAAVTAAAKGVLSEKLRSFSMQDLTLIRDEDALPLQRPDGRLRPSPGSLLDTIEDLGDDPALSLRSSTNPADSRTEASEDDMGDKAPKRAKPIKKAPKAEPLASKTLKTRPKKKTSGGGDSA 相同性解析による交差性が予測される生物種
Full Name	receptor accessory protein 2
Calculated molecular weight	252 aa, 28 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC006218
Gene Symbol	REEP2
Gene ID (NCBI)	51308
RRID	AB_2238326
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BRK0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
FC protocol for REEP2 antibody 15684-1-AP	Download protocol
IF protocol for REEP2 antibody 15684-1-AP	Download protocol
IHC protocol for REEP2 antibody 15684-1-AP	Download protocol
WB protocol for REEP2 antibody 15684-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature ER proteins decipher the tubulin code to regulate organelle distribution Authors - Pengli Zheng View Article
mouse	WB	Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. Authors - Xiao Wang View Article
mouse,human	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
mouse	IP	Mol Psychiatry Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease. Authors - M G Sharoar View Article
mouse	WB	Hum Mol Genet Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation. Authors - Benoît Renvoisé View Article
mouse	WB	Hum Mol Genet Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons. Authors - Peng-Peng Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15684-1-AP

WB analysis of human heart using 15684-1-AP

WB analysis of mouse eye using 15684-1-AP

WB analysis of human skeletal muscle using 15684-1-AP

IP experiment of mouse brain using 15684-1-AP

IHC staining of mouse brain using 15684-1-AP

IHC staining of mouse brain using 15684-1-AP

IF Staining of mouse brain using 15684-1-AP

FC experiment of SH-SY5Y using 15684-1-AP

REEP2 Polyclonal antibody

REEP2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

ER proteins decipher the tubulin code to regulate organelle distribution

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

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