Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10638-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 cells using 10638-1-AP
WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells.
WB analysis using 10638-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10638-1-AP
WB result of REDD1 specific antibody (10638-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REDD1 specific transfected K-562 cells.
WB analysis using 10638-1-AP
Non-treated Hela and Cobalt Chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 incubated at room temperature for 6 hours. .
WB analysis using 10638-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 10638-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10638-1-AP
IP result of anti-REDD1 specific (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug.
IHC staining of human lung cancer using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 10638-1-AP (REDD1 specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 10638-1-AP (REDD1 specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10638-1-AP (REDD1 specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10638-1-AP (REDD1 specific antibody) at dilution of 1:100 (under 40x lens).
IF Staining of LNCaP using 10638-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody (10638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10638-1-AP

Publications(285)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

DDIT4, REDD1, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, REDD-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (285)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, DU 145 cells, PC-3 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, K-562 cells, A549 cells, SH-SY5Y cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human lung cancer tissue, human heart tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	LNCaP cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 38 publications below
WB	See 264 publications below
IHC	See 25 publications below
IF	See 19 publications below
IP	See 1 publications below
CoIP	See 3 publications below
ChIP	See 2 publications below

Product Information

10638-1-AP targets REDD1 specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, rabbit, squirrel, gerbil
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0965 Product name: Recombinant human REDD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-232 aa of BC007714 Sequence: MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC 相同性解析による交差性が予測される生物種
Full Name	DNA-damage-inducible transcript 4
Calculated molecular weight	25 kDa
Observed molecular weight	32-35 kDa
GenBank accession number	BC007714
Gene Symbol	REDD1/DDIT4
Gene ID (NCBI)	54541
RRID	AB_2245711
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NX09
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

Protocols

Product Specific Protocols
IF protocol for REDD1 specific antibody 10638-1-AP	Download protocol
IHC protocol for REDD1 specific antibody 10638-1-AP	Download protocol
IP protocol for REDD1 specific antibody 10638-1-AP	Download protocol
WB protocol for REDD1 specific antibody 10638-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Plzf regulates germline progenitor self-renewal by opposing mTORC1. Authors - Hobbs Robin M RM View Article
human	WB	Cell Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR. Authors - Zandra E Walton View Article
mouse,human	WB	Cell Metab Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1 Authors - Kai Lin View Article
mouse	WB	Cancer Cell A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy. Authors - Isabelle K Vila View Article
rat	WB	Nat Med REDD1 is essential for stress-induced synaptic loss and depressive behavior. Authors - Kristie T Ota View Article
mouse	chIP	Nat Immunol The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. Authors - Wilson Castro View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10638-1-AP

WB analysis of PC-3 cells using 10638-1-AP

WB analysis using 10638-1-AP

WB analysis of K-562 using 10638-1-AP

WB analysis using 10638-1-AP

WB analysis using 10638-1-AP

WB analysis of DU 145 using 10638-1-AP

IP experiment of MCF-7 using 10638-1-AP

IHC staining of human lung cancer using 10638-1-AP

IHC staining of mouse brain using 10638-1-AP

IHC staining of mouse brain using 10638-1-AP

IHC staining of human liver using 10638-1-AP

IHC staining of human liver using 10638-1-AP

IHC staining of human heart using 10638-1-AP

IHC staining of human heart using 10638-1-AP

IF Staining of LNCaP using 10638-1-AP

REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Plzf regulates germline progenitor self-renewal by opposing mTORC1.

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1

A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

REDD1 is essential for stress-induced synaptic loss and depressive behavior.

The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.