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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RECQL5 Polyclonal antibody

RECQL5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12468-2-AP

Synonyms

ATP dependent DNA helicase Q5, DNA helicase, RecQ like type 5, RecQ protein like 5, RECQ5, RECQL5

Validation Data Gallery

Applications:
  • WB analysis using 12468-2-AP

    WB analysis using 12468-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 12468-2-AP

    WB analysis of mouse testis using 12468-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12468-2-AP

    IHC staining of human pancreas cancer using 12468-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12468-2-AP (RECQL5 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 12468-2-AP

    IF Staining of HepG2 using 12468-2-AP

    Immunofluorescent analysis of HepG2 cells, using RECQL5 antibody 12468-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHEK-293T cells, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12468-2-AP targets RECQL5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RECQL5 fusion protein Ag3145 相同性解析による交差性が予測される生物種
Full Name RecQ protein-like 5
Calculated molecular weight 435 aa, 49 kDa, 409 kDa
Observed molecular weight 48 kDa, 130-140 kDa
GenBank accession numberBC016911
Gene symbol RECQL5
Gene ID (NCBI) 9400
RRIDAB_2300899
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RECQL5 antibody 12468-2-APDownload protocol
IHC protocol for RECQL5 antibody 12468-2-APDownload protocol
IF protocol for RECQL5 antibody 12468-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols