Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | U-251 cells |
| Positive FC (Intra) detected in | Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85024-6-RR targets RECQL in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6834 Product name: Recombinant human RECQL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-448 aa of BC001052 Sequence: NVTMAGKEVFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFTFTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSLQNLGIHAGAYHANLEPEDKTTVHRKWSANEIQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDMKADCILYYGFGDIFRISSMVVMENVGQQKLYEMVSYCQ 相同性解析による交差性が予測される生物種 |
| Full Name | RecQ protein-like (DNA helicase Q1-like) |
| Calculated molecular weight | 73 kDa |
| GenBank accession number | BC001052 |
| Gene Symbol | RECQL |
| Gene ID (NCBI) | 5965 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P46063 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RECQL, also known as RecQ-like helicase, is a member of the RecQ DNA helicase family. These enzymes play a crucial role in maintaining genomic integrity by participating in various DNA repair processes, including mismatch repair, nucleotide excision repair, and direct repair. It's reported that the low RECQL expression is strongly associated with poor prognosis in breast cancer. RECQL expression may be a useful marker in estimating the prognosis of breast cancer patients. (PMID: 29914420)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RECQL antibody 85024-6-RR | Download protocol |
| IF protocol for RECQL antibody 85024-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |