Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 8 publications below |
Product Information
10793-1-AP targets REC8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1250 Product name: Recombinant human REC8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 188-548 aa of BC010887 Sequence: VLPPEAITILEAEPIRMLEIEGERELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELRLPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFRTPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSVPLMVSLEISLEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALELQANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH 相同性解析による交差性が予測される生物種 |
| Full Name | REC8 homolog (yeast) |
| Calculated molecular weight | 63 kDa |
| Observed molecular weight | 85-95 kDa |
| GenBank accession number | BC010887 |
| Gene Symbol | REC8 |
| Gene ID (NCBI) | 9985 |
| RRID | AB_2178282 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95072 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REC8 antibody 10793-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I | ||
Clin Cancer Res Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients. | ||
Ecotoxicol Environ Saf Impaired testicular development in male offspring following gestational DEHP exposure: The role of macrophage-Leydig cell axis. | ||
Int J Mol Sci "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. | ||
Biol Res REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells. | ||
Biomed Pharmacother Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway. |