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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RDX Polyclonal antibody (26105-1-AP)

    WB analysis using 26105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RDX Polyclonal antibody (26105-1-AP)

    WB analysis of mouse liver using 26105-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RDX Polyclonal antibody (26105-1-AP)

    WB analysis of HeLa using 26105-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RDX Polyclonal antibody (26105-1-AP)

    WB analysis of Jurkat using 26105-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Radixin Polyclonal antibody (26105-1-AP)

    IF Staining of HeLa using 26105-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26105-1-AP (RDX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Radixin Polyclonal antibody

Radixin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26105-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

RDX

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOS-7 cells, HeLa cells, Jurkat cells, mouse liver tissue, C6 cells, mouse brain tissue, mouse lung tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

26105-1-AP targets Radixin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23532

Product name: Recombinant human RDX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 384-455 aa of BC047109

Sequence: EEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAELAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQED

相同性解析による交差性が予測される生物種
Full Name radixin
Calculated molecular weight 583 aa, 68 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC047109
Gene Symbol RDX
Gene ID (NCBI) 5962
RRIDAB_2880380
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.

Protocols

Product Specific Protocols
IF protocol for Radixin antibody 26105-1-APDownload protocol
WB protocol for Radixin antibody 26105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Dev Cell

Generation of germinal-vesicle oocytes from mouse embryonic stem cells under an ovarian soma-free condition

Authors - Yoshiaki Nosaka
View Article
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