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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using RDM1 Polyclonal antibody (20156-1-AP)

    WB analysis of PC-3 using 20156-1-AP (same clone as 20156-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using RDM1 Polyclonal antibody (20156-1-AP)

    WB analysis of human placenta using 20156-1-AP (same clone as 20156-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RDM1 Polyclonal antibody (20156-1-AP)

    WB analysis of MCF-7 using 20156-1-AP (same clone as 20156-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using RDM1 Polyclonal antibody (20156-1-AP)

    IHC staining of human lung using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using RDM1 Polyclonal antibody (20156-1-AP)

    IHC staining of human lung using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using RDM1 Polyclonal antibody (20156-1-AP)

    IHC staining of human brain using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20156-1-AP (RDM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using RDM1 Polyclonal antibody (20156-1-AP)

    IHC staining of human brain using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20156-1-AP (RDM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RDM1 Polyclonal antibody (20156-1-AP)

    IHC staining of human testis using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 20156-1-AP (RDM1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RDM1 Polyclonal antibody (20156-1-AP)

    IHC staining of human testis using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 20156-1-AP (RDM1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using RDM1 Polyclonal antibody (20156-1-AP)

    IF Staining of PC-3 using 20156-1-AP (same clone as 20156-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 20156-1-AP (RDM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RDM1 Polyclonal antibody, PBS Only

RDM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20156-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20156-1-PBS targets RDM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14077

Product name: Recombinant human RDM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-253 aa of BC038301

Sequence: QHSLFTAFSQFGLLYSVRVFPNAAVAHPGFYAVIKFYSARAAHRAQKACDRKQLFQKSPVKVRLGTRHKAVQHQALALNSSKCQELANYCFGFNGCSKRIIKLQELSDLEERENEDSMVPLPKQSLKFFCALEVVLPSCDCRSPGIGLVEEPMDKVEEGPLSFLMKRKTAQKLAIQKALSDAFQKLLIVVLESGKIAVEYRPSEDIVGVRCEEELHGLIQVPCSPWKQYGQEEEGYLSDFSLEEEEFRLPELD

相同性解析による交差性が予測される生物種
Full Name RAD52 motif 1
Calculated molecular weight 284 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC038301
Gene Symbol RDM1
Gene ID (NCBI) 201299
RRIDAB_10666433
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG50
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)

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