Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13289-3-AP targets RDH12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3850 Product name: Recombinant human RDH12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC025724 Sequence: MLVTLGLLTSFFSFLYMVAPSIRKFFAGGVCRTNVQLPGKVVVITGANTGIGKETARELASRGARVYIACRDVLKGESAASEIRVDTKNSQVLVRKLDLSDTKSIRAFAEGFLAEEKQLHILINNAGVMMCPYSKTADGFETHLGVNHLGHFLLTYLLLEQLKVSAPARVVNVSSVAHHIGKIPFHDLQSEKRYSRGFAYCHSKLANVLFTRELAKRLQGTGVTTYAVHPGVVRSELVRHSSLLCLLWRLFSPFVKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRTWVSPRARNNKTAERLWNVSCELLGIRWE 相同性解析による交差性が予測される生物種 |
| Full Name | retinol dehydrogenase 12 (all-trans/9-cis/11-cis) |
| Calculated molecular weight | 316 aa, 35 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC025724 |
| Gene Symbol | RDH12 |
| Gene ID (NCBI) | 145226 |
| RRID | AB_2178131 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Retinol dehydrogenase 12 (RDH12) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily. This NADPH-dependent enzyme is highly expressed in the photoreceptor inner segments of the retina, where it functions as a key component of the visual cycle. RDH12 catalyzes the reduction of all-trans-retinal to all-trans-retinol, a critical step in regenerating the visual chromophore 11-cis-retinal. Mutations in the RDH12 gene cause autosomal recessive retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA), severe inherited retinal dystrophies characterized by early-onset vision loss and photoreceptor degeneration. Loss of RDH12 activity leads to accumulation of toxic all-trans-retinal, triggering oxidative stress and cell death. RDH12 also exhibits retinoid-binding properties and may play additional roles in lipid metabolism and cellular stress responses beyond its enzymatic function in the visual cycle.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RDH12 antibody 13289-3-AP | Download protocol |
| WB protocol for RDH12 antibody 13289-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |