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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RDH11 Polyclonal antibody

RDH11 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11077-1-AP

Synonyms

ARSDR1, CGI 82, HCBP12, MDT1, PSDR1, RALR1, RDH11, Retinal reductase 1, Retinol dehydrogenase 11, SCALD, SDR7C1

Validation Data Gallery

Applications:
  • IHC staining of human prostate cancer using 11077-1-AP

    IHC staining of human prostate cancer using 11077-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11077-1-AP (RDH11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 11077-1-AP

    IHC staining of human prostate cancer using 11077-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11077-1-AP (RDH11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 11077-1-AP

    IF Staining of PC-3 using 11077-1-AP

    Immunofluorescent analysis of PC-3 cells using 11077-1-AP (RDH11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11077-1-AP targets RDH11 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDH11 fusion protein Ag1485 相同性解析による交差性が予測される生物種
Full Name retinol dehydrogenase 11 (all-trans/9-cis/11-cis)
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC011727
Gene symbol RDH11
Gene ID (NCBI) 51109
RRIDAB_2177870
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RDH11(Retinol dehydrogenase 11) is also named as ARSDR1(androgen-regulated short-chain dehydrogenase/reductase 1 ), PSDR1(prostate short-chain dehydrogenase/reductase 1) and belongs to the short-chain dehydrogenases/reductases (SDR) family. RDH11 is involved in steroid synthesis and/or degradation in normal and neoplastic prostate epithelium, and, as such, it may be a key enzyme involved in maintaining intracellular balance of steroid hormones in these cells(PMID:11245473). The deduced protein contains 318 amino acids, and the transcript contains 2 potential polyadenylation signals. It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IHC protocol for RDH11 antibody 11077-1-APDownload protocol
IF protocol for RDH11 antibody 11077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols