Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, MOLT-4 cells, Neuro-2a cells, C6 cells, PC-12 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86954-1-RR targets RCOR1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag26770 Product name: Recombinant human RCOR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 245-310 aa of NM_015156 Sequence: MRHARKQKREREESEDELEEANGNNPIDIEVDQNKESKKEVPPTETVPQVKKEKHSTQAKNRAKRKP 相同性解析による交差性が予測される生物種 |
| Full Name | REST corepressor 1 |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | NM_015156 |
| Gene Symbol | RCOR1 |
| Gene ID (NCBI) | 23186 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UKL0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RCOR1, also named as REST corepressor 1, is a 485 amino acid protein, which belongs to the CoREST family. RCOR1 is a essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. The calculated molecular weight of RCOR1 is a 53 kDa, but the phosphorylated RCOR1 is about 60-65 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RCOR1 antibody 86954-1-RR | Download protocol |
| WB protocol for RCOR1 antibody 86954-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |