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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCCD1 Polyclonal antibody (24446-1-AP)

    WB analysis using 24446-1-AP (same clone as 24446-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24446-1-AP (RCCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RCCD1 Polyclonal antibody (24446-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP (same clone as 24446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RCCD1 Polyclonal antibody (24446-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP (same clone as 24446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RCCD1 Polyclonal antibody (24446-1-AP)

    IHC staining of human stomach using 24446-1-AP (same clone as 24446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24446-1-AP (RCCD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RCCD1 Polyclonal antibody (24446-1-AP)

    IHC staining of human stomach using 24446-1-AP (same clone as 24446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24446-1-AP (RCCD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24446-1-PBS in a different storage buffer formulation.

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RCCD1 Polyclonal antibody, PBS Only

RCCD1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24446-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

RCC1 domain-containing protein 1, RCC1 domain containing 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24446-1-PBS targets RCCD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCCD1 fusion protein Ag19685 相同性解析による交差性が予測される生物種
Full Name RCC1 domain containing 1
Calculated molecular weight 376 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC140708
Gene Symbol RCCD1
Gene ID (NCBI) 91433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NED2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RCCD1 is a new member of the large, phylogenetically conserved, RCC1 superfamily. Many RCC1 superfamily proteins have been linked to several human diseases, including cancer. RCCD1 was reported as a partner of histone H3K36 demethylase KDM8 involved in chromosome segregation, and has been identified as a potential driver for breast cancer. RCCD1 plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial-to-mesenchymal transition (EMT), potentially through the inhibition of KDM8.