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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBPMS Recombinant antibody (85086-5-RR)

    WB analysis using 85086-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85086-5-RR (RBPMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RBPMS Recombinant antibody (85086-5-RR)

    IHC staining of mouse eye using 85086-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RBPMS Recombinant antibody (85086-5-RR)

    IHC staining of mouse eye using 85086-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse retina tissue using RBPMS Recombinant antibody (85086-5-RR)

    IF Staining of mouse retina using 85086-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBPMS Recombinant antibody (85086-5-RR)

    IF Staining of HepG2 using 85086-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using RBPMS Recombinant antibody (85086-5-RR)

    FC experiment of A549 using 85086-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug RBPMS Recombinant antibody (85086-5-RR, Clone:242730B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85086-5-RR

    Affinity and Kinetic Characterization of 85086-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85086-5-RR against Human RBPMS were performed. The affinity constant is 0.153 nM.

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RBPMS Recombinant antibody

RBPMS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 85086-5-RR
Clone No.242730B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

242730B7, Hermes, RBP MS, RBP-MS, RNA-binding protein with multiple splicing

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, SKOV-3 cells, MDA-MB-231 cells, A549 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse retina tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85086-5-RR targets RBPMS in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBPMS fusion protein Ag7256 相同性解析による交差性が予測される生物種
Full Name RNA binding protein with multiple splicing
Calculated molecular weight 22 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC003608
Gene Symbol RBPMS
Gene ID (NCBI) 11030
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.

Protocols

Product Specific Protocols
WB protocol for RBPMS antibody 85086-5-RRDownload protocol
IHC protocol for RBPMS antibody 85086-5-RRDownload protocol
IF protocol for RBPMS antibody 85086-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols