Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, mouse kidney tissue, mouse liver tissue, mouse colon tissue, mouse heart tissue, rat colon tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat eye tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 6 publications below |
| IF | See 26 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
15187-1-AP targets RBPMS in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7256 Product name: Recombinant human RBPMS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC003608 Sequence: MNNGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDSRSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPALYPSSPEVWAPYPLYPAELAPALPPPAFTYPASLHAQCFSPEAKPNTPVFCPLLQQIRFVSGNVFVTYQPTADQQRELPC 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding protein with multiple splicing |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 28-30 kDa |
| GenBank accession number | BC003608 |
| Gene Symbol | RBPMS |
| Gene ID (NCBI) | 11030 |
| RRID | AB_2238431 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q93062 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RBPMS antibody 15187-1-AP | Download protocol |
| IF protocol for RBPMS antibody 15187-1-AP | Download protocol |
| IHC protocol for RBPMS antibody 15187-1-AP | Download protocol |
| IP protocol for RBPMS antibody 15187-1-AP | Download protocol |
| WB protocol for RBPMS antibody 15187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Perivascular neurons instruct 3D vascular lattice formation via neurovascular contact | ||
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Cell Rep Comparative transcriptomic profiling reveals a role for Olig1 in promoting axon regeneration | ||
Cell Rep Pan-cancer tRNA-derived fragment CAT1 coordinates RBPMS to stabilize NOTCH2 mRNA to promote tumorigenesis | ||
Cell Rep Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina. | ||
Sci Rep Develop an efficient and specific AAV-based labeling system for Muller glia in mice |
