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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RBPJ Monoclonal antibody

RBPJ Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F12G12

Cat no : CL488-66132

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-66132

    FC experiment of HeLa using CL488-66132

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RBPJ (CL488-66132, Clone:2F12G12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RBPJ Ready-To-Use IHC Kit. RBPJ primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66132-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66132 is RBPJ Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBPJ fusion protein Ag6302 相同性解析による交差性が予測される生物種
Full Name recombination signal binding protein for immunoglobulin kappa J region
Calculated molecular weight 56 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC064976
Gene symbol RBPJ
Gene ID (NCBI) 3516
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 RBPJ antibody CL488-66132Download protocol
Standard Protocols
Click here to view our Standard Protocols