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Validation Data Gallery

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  • Western Blot (WB) analysis of pig kidney tissue using RBP5 Monoclonal antibody (68126-1-Ig)

    WB analysis of pig kidney using 68126-1-Ig (same clone as 68126-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using RBP5 Monoclonal antibody (68126-1-Ig)

    WB analysis of pig liver using 68126-1-Ig (same clone as 68126-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBP5 Monoclonal antibody (68126-1-Ig)

    WB analysis using 68126-1-Ig (same clone as 68126-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBP5 Monoclonal antibody (68126-1-Ig)

    IF Staining of HepG2 using 68126-1-Ig (same clone as 68126-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBP5 Monoclonal antibody (68126-1-Ig)

    IF Staining of A431 using 68126-1-Ig (same clone as 68126-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

RBP5 Monoclonal antibody, PBS Only

RBP5 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68126-1-PBS
Clone No.1G7E2

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68126-1-PBS targets RBP5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24562

Product name: Recombinant human RBP5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-135 aa of BC029355

Sequence: MPPNLTGYYRFVSQKNMEDYLQALNISLAVRKIALLLKPDKEIEHQGNHMTVRTLSTFRNYTVQFDVGVEFEEDLRSVDGRKCQTIVTWEEEHLVCVQKGEVPNRGWRHWLEGEMLYLELTARDAVCEQVFRKVR

相同性解析による交差性が予測される生物種
Full Name retinol binding protein 5, cellular
Calculated molecular weight 135 aa, 16 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberBC029355
Gene Symbol RBP5
Gene ID (NCBI) 83758
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP82980
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).

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