Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse eye tissue, Y79 cells |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
14352-1-AP targets RBP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5699 Product name: Recombinant human RBP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 898-1247 aa of BC039844 Sequence: AMSATTGKAWDLAGVEPDITVPMSEALSIAQDIVALRAKVPTVLQTAGKLVADNYASAELGAKMATKLSGLQSRYSRVTSEVALAEILGADLQMLSGDPHLKAAHIPENAKDRIPGIVPMQIPSPEVFEELIKFSFHTNVLEDNIGYLRFDMFGDGELLTQVSRLLVEHIWKKIMHTDAMIIDMRFNIGGPTSSIPILCSYFFDEGPPVLLDKIYSRPDDSVSELWTHAQVVGERYGSKKSMVILTSSVTAGTAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTNLYLTIPTARSVGASDGSSWEGVGVTPHVVVPAEEALARAKEMLQHNQLRVKRSPGLQDHL 相同性解析による交差性が予測される生物種 |
| Full Name | retinol binding protein 3, interstitial |
| Calculated molecular weight | 135 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC039844 |
| Gene Symbol | RBP3 |
| Gene ID (NCBI) | 5949 |
| RRID | AB_2096956 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10745 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBP3 (retinoid-binding protein 3), also called interstitial retinoid-binding protein (IRBP), is a large glycoprotein that is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix. It is known to bind retinoids and fatty acids. RBP3 migrates around 136-140 kDa in the western blot analysis. (PMID: 15928609)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBP3 antibody 14352-1-AP | Download protocol |
| IP protocol for RBP3 antibody 14352-1-AP | Download protocol |
| WB protocol for RBP3 antibody 14352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med Identification of a novel cis-regulatory element essential for immune tolerance. | ||
Acta Biomater Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. | ||
Proc Natl Acad Sci U S A Development of a therapeutic anti-HtrA1 antibody and the identification of DKK3 as a pharmacodynamic biomarker in geographic atrophy. | ||
Diabetes Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-induced Retinal Dysfunction and Neurodegeneration through Rhodopsin. | ||
iScience A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. | ||
Sci Rep Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation |
