Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse small intestine tissue, rat small intestine tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31527-1-AP targets RBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35801 Product name: Recombinant human RBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-134 aa of NM_004164.2 Sequence: RKIAVRLTQTKVIDQDGDNFKTKTTSTFRNYDVDFTVGVEFDEYTKSLDNRHVKALVTWEGDVLVCVQKGEKENRGWKQWIEGDKLYLELTCGDQVCRQVFKKK 相同性解析による交差性が予測される生物種 |
| Full Name | retinol binding protein 2, cellular |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | NM_004164.2 |
| Gene Symbol | RBP2 |
| Gene ID (NCBI) | 5948 |
| RRID | AB_3670022 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P50120 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Retinol-binding protein 2 (RBP2) also known as CRBP2, CRBP-II, is an abundant 134-residue protein confined largely to the small intestinal enterocyte. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A(PMID: 3029082).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBP2 antibody 31527-1-AP | Download protocol |
| WB protocol for RBP2 antibody 31527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |