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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RBP2 Polyclonal antibody (18825-1-AP)

    WB analysis of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using RBP2 Polyclonal antibody (18825-1-AP)

    WB analysis of HEK-293T using 18825-1-AP (same clone as 18825-1-PBS)

    WB result of KDM5A antibody (18825-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and KDM5A knockout HEK-293T cells. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBP2 Polyclonal antibody (18825-1-AP)

    IP experiment of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using RBP2 Polyclonal antibody (18825-1-AP)

    IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using RBP2 Polyclonal antibody (18825-1-AP)

    IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBP2 Polyclonal antibody (18825-1-AP)

    IF Staining of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RBP2 Polyclonal antibody, PBS Only

RBP2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 18825-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18825-1-PBS targets RBP2 in WB, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 5A
Calculated molecular weight 192 kDa
Observed molecular weight 192 kDa
GenBank accession numberNM_005056
Gene Symbol KDM5A
Gene ID (NCBI) 5927
RRIDAB_10638436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29375
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.