Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 22683-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 22683-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 22683-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22683-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 22683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 22683-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RBP1 antibody (22683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

RBP1 Polyclonal antibody

RBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22683-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cellular retinol-binding protein, Cellular retinol-binding protein I, CRABP I, CRBP I, CRBP1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, HEK-293 cells, human brain tissue, human liver tissue, rat liver tissue
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue, human liver tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

22683-1-AP targets RBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18572 Product name: Recombinant human RBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC121052 Sequence: MPVDFTGYWKMLVNENFEEYLRALDVNVALRKIANLLKPDKEIVQDGDHMIIRTLSTFRNYIMDFQVGKEFEEDLTGIDDRKCMTTVSWDGDKLQCVQKGEKEGRGWTQWIEGDELHLEMRVEGVVCKQVFKKVQ 相同性解析による交差性が予測される生物種
Full Name	retinol binding protein 1, cellular
Calculated molecular weight	135 aa, 16 kDa
Observed molecular weight	14-21 kDa
GenBank accession number	BC121052
Gene Symbol	RBP1
Gene ID (NCBI)	5947
RRID	AB_11182381
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09455
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RBP1 antibody 22683-1-AP	Download protocol
IHC protocol for RBP1 antibody 22683-1-AP	Download protocol
WB protocol for RBP1 antibody 22683-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Metabolism Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved. Authors - Shyi-Jang Shin View Article
human	WB,IHC	Transl Oncol Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. Authors - Xinlu Liu View Article
rat	IHC	Brain Sci Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus. Authors - Tianjia Li View Article
mouse	WB	J Food Biochem Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice. Authors - Wei Li View Article
human	WB,IHC,IF	Cell Death Dis The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma. Authors - Ling Gao KD Validated View Article
human	WB	FASEB J The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation Authors - Dan-Dan Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22683-1-AP

WB analysis of human brain using 22683-1-AP

WB analysis of human liver using 22683-1-AP

WB analysis of HEK-293 using 22683-1-AP

IHC staining of human colon cancer using 22683-1-AP

IHC staining of human colon cancer using 22683-1-AP

IHC staining of human liver using 22683-1-AP

IHC staining of human liver using 22683-1-AP

IHC staining of human liver cancer using 22683-1-AP

IHC staining of human liver cancer using 22683-1-AP

IHC staining of human ovary using 22683-1-AP

IHC staining of human ovary using 22683-1-AP

IF Staining of HEK-293 using 22683-1-AP

RBP1 Polyclonal antibody

RBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus.

Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.

The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.

The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation