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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis using 14958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis using 14958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of HeLa using 14958-1-AP

    WB result of RBM8A,Y14 antibody (14958-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of HeLa using 14958-1-AP

    WB result of RBM8A,Y14 antibody (14958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of HeLa using 14958-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of Jurkat cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    WB analysis of Jurkat using 14958-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IP experiment of HeLa using 14958-1-AP

    IP result of anti-RBM8A,Y14 (IP:14958-1-AP, 4ug; Detection:14958-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human brain using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human spleen using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human spleen using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human ovary using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human ovary using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human testis using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human testis using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IHC staining of human brain using 14958-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IF Staining of HeLa using 14958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IF Staining of HeLa using 14958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM8A,Y14 Polyclonal antibody (14958-1-AP)

    IF Staining of HeLa using 14958-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

RBM8A,Y14 Polyclonal antibody

RBM8A,Y14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14958-1-AP
Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RBM8A, BOV-1, BOV 1C, BOV 1B, BOV 1A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells, MDA-MB-453s cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human spleen tissue, human testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14958-1-AP targets RBM8A,Y14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6800

Product name: Recombinant human RBM8A,Y14 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-174 aa of BC017088

Sequence: MADVLDLHEAGGEDFAMDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARMREDYDSVEQDGDEPGPQRSVEGWILFVTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYETYKEAQAAMEGLNGQDLMGQPISVDWCFVRGPPKGKRRGGRRRSRSPDRRRR

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 8A
Calculated molecular weight 20 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC017088
Gene Symbol RBM8A
Gene ID (NCBI) 9939
RRIDAB_2178820
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5S9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IHC protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
IP protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
WB protocol for RBM8A,Y14 antibody 14958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
View Article
mouseIF

RNA

An atlas of chromatoid body components.

Authors - Oliver Meikar
View Article
humanWB,IHC

Front Oncol

RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.

Authors - Yan Lin
  • KD Validated
View Article
humanWB

Cell Death Discov

CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.

Authors - Xiaojin Song
View Article
humanWB,IF

Cell Rep

The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

Authors - Fernando C Alsina
View Article
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