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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HeLa using 21896-1-AP (same clone as 21896-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RBM7 Polyclonal antibody (21896-1-AP)

    WB analysis of HeLa using 21896-1-AP (same clone as 21896-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human liver cancer using 21896-1-AP (same clone as 21896-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human lung cancer using 21896-1-AP (same clone as 21896-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human liver cancer using 21896-1-AP (same clone as 21896-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RBM7 Polyclonal antibody (21896-1-AP)

    IHC staining of human lung cancer using 21896-1-AP (same clone as 21896-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM7 Polyclonal antibody (21896-1-AP)

    IF Staining of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM7 antibody (21896-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

RBM7 Polyclonal antibody, PBS Only

RBM7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21896-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21896-1-PBS targets RBM7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11106

Product name: Recombinant human RBM7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 5-266 aa of BC034381

Sequence: AAEADRTLFVGNLETKVTEELLFELFHQAGPVIKVKIPKDKDGKPKQFAFVNFKHEVSVPYAMNLLNGIKLYGRPIKIQFRSGSSHAPQDVSLSYPQHHVGNSSPTSTSPSRYERTMDNMTSSAQIIQRSFSSPENFQRQAVMNSALRQMSYGGKFGSSPLDQSGFSPSVQSHSHSFNQSSSSQWRQGTPSSQRKVRMNSYPYLADRHYSREQRYTDHGSDHHYRGKRDDFFYEDRNHDDWSHDYDNRRDSSRDGKWRSSRH

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 7
Calculated molecular weight 266 aa, 31 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC034381
Gene Symbol RBM7
Gene ID (NCBI) 10179
RRIDAB_2878938
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y580
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).

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