Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human stomach tissue, human lung tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
19930-1-AP targets RBM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RBM5 fusion protein Ag13908 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 5 |
| Calculated molecular weight | 815 aa, 92 kDa |
| Observed molecular weight | 110-115 kDa |
| GenBank accession number | BC002957 |
| Gene Symbol | RBM5 |
| Gene ID (NCBI) | 10181 |
| RRID | AB_2878625 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52756 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM5, also named as RNA-binding protein 5, is a 815 amino acid protein, which contains 2 RRM (RNA recognition motif) domains and Belongs to the RBM5/RBM10 family. RBM5 localizes in the nucleus and is a component of the spliceosome A complex. RBM5 regulates alternative splicing of a number of mRNAs and may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. The phosphorylation of RBM5 protein would influence the detected molecular weight. A 105-110 KDa band was also reported (PMID: 27957556).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM5 antibody 19930-1-AP | Download protocol |
| IHC protocol for RBM5 antibody 19930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem CHIP suppresses the proliferation and migration of A549 cells by mediating the ubiquitination of eIF2α and upregulation of tumor suppressor RBM5 | ||
J Neurotrauma RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but not Hippocampal Cell Death. | ||
Cell Death Discov Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent manner |