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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM47 Recombinant antibody (86009-1-RR)

    WB analysis using 86009-1-RR (same clone as 86009-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86009-1-RR (RBM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM47 Recombinant antibody (86009-1-RR)

    IHC staining of human colon using 86009-1-RR (same clone as 86009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM47 Recombinant antibody (86009-1-RR)

    IHC staining of human colon using 86009-1-RR (same clone as 86009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RBM47 Recombinant antibody (86009-1-RR)

    IF Staining of A549 using 86009-1-RR (same clone as 86009-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM47 antibody (86009-1-RR, Clone: 250436H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86009-1-RR

    Affinity and Kinetic Characterization of 86009-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86009-1-RR against Human RBM47 were performed. The affinity constant is 1.02 nM.

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RBM47 Recombinant antibody, PBS Only

RBM47 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86009-1-PBS
Clone No.250436H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NET18, RNA binding motif protein 47, RNA binding protein 47, RNA-binding motif protein 47, RNA-binding protein 47

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86009-1-PBS targets RBM47 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM47 fusion protein Ag20984 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 47
Calculated molecular weight 593 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC126261
Gene Symbol RBM47
Gene ID (NCBI) 54502
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDA0AV96
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM47, also known as RNA-binding motif protein 47 or RNA-binding protein 47, is a vertebrate-conserved RBP containing three RNA-recognition motifs (RRMs) that perform multifaceted roles in RNA editing, early embryonic development, and cancer.Recent studies suggest that RBM47 functions as a tumor suppressor. It has been shown to suppress the progression of breast and colon cancers. RBM47 represents a molecular switch of cell fate decisions that functions as a regulator of the p53/p21-signaling axis. (PMID: 34499322, PMID: 31511650)