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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RBM42 Polyclonal antibody (15407-1-AP)

    WB analysis of HepG2 using 15407-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM42 Polyclonal antibody (15407-1-AP)

    WB analysis of HeLa using 15407-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RBM42 Polyclonal antibody (15407-1-AP)

    WB analysis of NIH/3T3 using 15407-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using RBM42 Polyclonal antibody (15407-1-AP)

    WB analysis of human liver using 15407-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RBM42 Polyclonal antibody (15407-1-AP)

    WB analysis of HEK-293 using 15407-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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RBM42 Polyclonal antibody

RBM42 Polyclonal Antibody for WB, ELISA
Cat No. 15407-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RNA-binding protein 42, RNA-binding motif protein 42, RBM 42

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, human liver tissue, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15407-1-AP targets RBM42 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM42 fusion protein Ag7667 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 42
Calculated molecular weight 50 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC004204
Gene Symbol RBM42
Gene ID (NCBI) 79171
RRIDAB_2176113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM42, also known as RNA-binding motif protein 42, is a member of the RNA-binding protein family that plays critical roles in post-transcriptional regulation of gene expression. Emerging studies highlight RBM42's involvement in pre-mRNA splicing through its association with the spliceosome complex, where it contributes to the precise removal of introns and assembly of mature mRNA. Beyond splicing, RBM42 has been implicated in cellular stress responses, cell cycle regulation, and apoptosis. Dysregulation of RBM42 is linked to developmental disorders and cancers; for instance, mutations in RBM42 are associated with neurodevelopmental abnormalities, while its overexpression or loss correlates with tumor progression in certain malignancies.

Protocols

Product Specific Protocols
WB protocol for RBM42 antibody 15407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols