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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb® RBM25 Multi-Recombinant antibody (RMX00066)

    WB analysis using RMX00066

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00066 (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Multi-rAb® RBM25 Multi-Recombinant antibody (RMX00066)

    WB analysis using RMX00066

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00066 (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Multi-rAb® RBM25 Multi-Recombinant antibody (RMX00066)

    IP experiment of HEK-293 using RMX00066

    IP result of anti-RBM25 (IP:RMX00066, 4ug; Detection:RMX00066 1:5000) with HEK-293 cells lysate 1000 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Multi-rAb® RBM25 Multi-Recombinant antibody (RMX00066)

    IHC staining of human colon cancer using RMX00066

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00066 (RBM25 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Multi-rAb® RBM25 Multi-Recombinant antibody (RMX00066)

    IHC staining of human colon cancer using RMX00066

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00066 (RBM25 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Multi-rAb® RBM25 Multi-Recombinant antibody (RMX00066)

    IF Staining of HeLa using RMX00066

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (RMX00066) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® RBM25 Multi-Recombinant antibody

RBM25 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00066

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25, RED120

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, PC-12 cells, Jurkat cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00066 targets RBM25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 25
Calculated molecular weight 843 aa, 100 kDa
Observed molecular weight100-120 kDa
GenBank accession numberBC113389
Gene Symbol RBM25
Gene ID (NCBI) 58517
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDP49756
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

Protocols

Product Specific Protocols
IF protocol for RBM25 antibody RMX00066Download protocol
IHC protocol for RBM25 antibody RMX00066Download protocol
IP protocol for RBM25 antibody RMX00066Download protocol
WB protocol for RBM25 antibody RMX00066Download protocol
Standard Protocols
Click here to view our Standard Protocols
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