• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RBM25 Recombinant antibody (84968-4-RR)

    WB analysis using 84968-4-RR (same clone as 84968-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84968-4-RR (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RBM25 Recombinant antibody (84968-4-RR)

    IHC staining of human colon cancer using 84968-4-RR (same clone as 84968-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84968-4-RR (RBM25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RBM25 Recombinant antibody (84968-4-RR)

    IHC staining of human colon cancer using 84968-4-RR (same clone as 84968-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84968-4-RR (RBM25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM25 Recombinant antibody (84968-4-RR)

    IF Staining of HeLa using 84968-4-RR (same clone as 84968-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (84968-4-RR, Clone: 242526B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84968-4-RR

    Affinity and Kinetic Characterization of 84968-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84968-4-RR against Human RBM25 were performed. The affinity constant is 2.38 nM.

RBM25 Recombinant monoclonal antibody, PBS Only

RBM25 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84968-4-PBS
Clone No.242526B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242526B7, Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84968-4-PBS targets RBM25 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18244

Product name: Recombinant human RBM25 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 605-839 aa of BC113389

Sequence: QKPCLKPTLRPISSAPSVSSASGNATPNTPGDESPCGIIIPHENSPDQQQPEEHRPKIGLSLKLGASNSPGQPNSVKRKKLPVDSVFNKFEDEDSDDVPRKRKLVPLDYGEDDKNATKGTVNTEEKRKHIKSLIEKIPTAKPELFAYPLDWSIVDSILMERRIRPWINKKIIEYIGEEEATLVDFVCSKVMAHSSPQSILDDVAMVLDEEAEVFIVKMWRLLIYETEAKKIGLVK

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 25
Calculated molecular weight 843 aa, 100 kDa
Observed molecular weight110-120 kDa
GenBank accession numberBC113389
Gene Symbol RBM25
Gene ID (NCBI) 58517
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP49756
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

||
New chat Able
正在加载，请稍候...