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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis of Raji using 22103-1-AP (same clone as 22103-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis using 22103-1-AP (same clone as 22103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis of NIH/3T3 using 22103-1-AP (same clone as 22103-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using RBM22 Polyclonal antibody (22103-1-AP)

    IHC staining of rat stomach using 22103-1-AP (same clone as 22103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22103-1-AP (RBM22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RBM22 Polyclonal antibody (22103-1-AP)

    IF Staining of U2OS using 22103-1-AP (same clone as 22103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

RBM22 Polyclonal antibody, PBS Only

RBM22 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22103-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

199G4, fSAP47, Pre mRNA splicing factor RBM22, Pre-mRNA-splicing factor RBM22, RNA binding motif protein 22

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22103-1-PBS targets RBM22 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17315

Product name: Recombinant human RBM22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-420 aa of BC003402

Sequence: MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLGDTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNVKWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAVVNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 22
Calculated molecular weight 420 aa, 47 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC003402
Gene Symbol RBM22
Gene ID (NCBI) 55696
RRIDAB_2878996
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NW64
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)

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