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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of rat heart tissue using RBM20 Polyclonal antibody (31177-1-AP)

    IHC staining of rat heart using 31177-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RBM20 Polyclonal antibody (31177-1-AP)

    IHC staining of mouse heart using 31177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RBM20 Polyclonal antibody

RBM20 Polyclonal Antibody for IHC, ELISA
Cat No. 31177-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

RNA binding protein, RNA binding motif protein 20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inrat heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31177-1-AP targets RBM20 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM20 fusion protein Ag34322 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 20
Calculated molecular weight134 kDa
GenBank accession numberNM_001134363
Gene Symbol RBM20
Gene ID (NCBI) 282996
RRIDAB_3669884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5T481
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM20 (RNA binding motif protein 20) is a striated muscle-specific splicing factor that regulates alternative splicing of many cardiac genes encoding sarcomere and calcium-regulatory proteins (PMID: 36417486). RBM20 has been identified as a crucial RNA-binding protein that controls the splicing of TTN (PMID: 30547036). RBM20 is expressed in all striated muscles but is highest in cardiac tissue (PMID: 34575212).

Protocols

Product Specific Protocols
IHC protocol for RBM20 antibody 31177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols