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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using RBM19 Polyclonal antibody (19095-1-AP)

    WB analysis of mouse colon using 19095-1-AP (same clone as 19095-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using RBM19 Polyclonal antibody (19095-1-AP)

    WB analysis of COLO 320 using 19095-1-AP (same clone as 19095-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using RBM19 Polyclonal antibody (19095-1-AP)

    WB analysis of mouse small intestine using 19095-1-AP (same clone as 19095-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM19 Polyclonal antibody (19095-1-AP)

    IHC staining of human colon using 19095-1-AP (same clone as 19095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM19 Polyclonal antibody (19095-1-AP)

    IHC staining of human colon using 19095-1-AP (same clone as 19095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RBM19 Polyclonal antibody (19095-1-AP)

    IF Staining of MCF-7 using 19095-1-AP (same clone as 19095-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using RBM19 antibody 19095-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19095-1-PBS in a different storage buffer formulation.

RBM19 Polyclonal antibody, PBS Only

RBM19 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19095-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19095-1-PBS targets RBM19 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13545

Product name: Recombinant human RBM19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC006137

Sequence: MSRLIVKNLPNGMKEERFRQLFAAFGTLTDCSLKFTKDGKFRKFGFIGFKSEEEAQKAQKHFNKSFIDTSRITVEFCKSFGDPAKPRAWSKHAQKPSQPKQPPKDSTTPEIKKDEKKKKVAGQLEKLKEDTEFQEFLSVHQRRAQAATWANDGLDAEPSKGKSKPASDYLNFDSDSGQESEEEGAGEDLEEEASLEPKAAVQKELSDMDYLKSKMVKAGSSSSSEEEESEDEAVHCDEGSEAEEEDSSATPVLQERDSRGAGQEQGMPAGKKRPPEARAETEKPANQKEPTTCHTVKLRG

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 19
Calculated molecular weight 107 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006137
Gene Symbol RBM19
Gene ID (NCBI) 9904
RRIDAB_10640906
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4C8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM19, also named as KIAA0682, is a 960 amino acid protein, which belongs to the RRM MRD1 family. RBM19 is expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia. RBM19 plays a role in embryo pre-implantation development. The calculated molecular weight of RBM19 is 107 kDa, but the modified RBM19 is about 120 kDa.

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