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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM17 Recombinant antibody

RBM17 Recombinant Antibody for IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230125A10

Cat no : 82889-2-RR

Synonyms

45 kDa splicing factor, DKFZp686F13131, RBM17, RNA binding motif protein 17, SPF45, Splicing factor 45

Validation Data Gallery

Applications:
  • IHC staining of mouse testis using 82889-2-RR

    IHC staining of mouse testis using 82889-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 82889-2-RR

    IHC staining of mouse testis using 82889-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82889-2-RR

    IF Staining of HeLa using 82889-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM17 antibody (82889-2-RR, Clone: 230125A10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 82889-2-RR

    FC experiment of A431 using 82889-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human RBM17 (82889-2-RR, Clone:230125A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:150-1:600
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

82889-2-RR targets RBM17 in IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM17 fusion protein Ag0985 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 17
Calculated molecular weight 45 kDa
GenBank accession numberBC007871
Gene symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

Protocols

Product Specific Protocols
IHC protocol for RBM17 antibody 82889-2-RRDownload protocol
IF protocol for RBM17 antibody 82889-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols