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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis of K-562 using 67506-1-Ig (same clone as 67506-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis of COLO 320 using 67506-1-Ig (same clone as 67506-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM15B Monoclonal antibody (67506-1-Ig)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using RBM15B Monoclonal antibody (67506-1-Ig)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

RBM15B Monoclonal antibody, PBS Only

RBM15B Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67506-1-PBS
Clone No.1C2C11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1C2C11, HsOTT3, HUMAGCGB, HuOTT3, One-twenty two protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67506-1-PBS targets RBM15B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17853

Product name: Recombinant human RBM15B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 490-769 aa of BC001367

Sequence: AKAEETRYPQQYQPSPLPVHYELLTDGYTRHRNLDADLVRDRTPPHLLYSDRDRTFLEGDWTSPSKSSDRRNSLEGYSRSVRSRSGERWGADGDRGLPKPWEERRKRRSLSSDRGRTTHSPYEERSRTKGSGQQSERGSDRTPERSRKENHSSEGTKESSSNSLSNSRHGAEERGHHHHHHEAADSSHGKKARDSERNHRTTEAEPKPLEEPKHETKKLKNLSEYAQTLQLGWNGLLVLKNSCFPTSMHILEGDQGVISSLLKDHTSGSKLTQLKIAQRL

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 15B
Calculated molecular weight 890 aa, 97 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC001367
Gene Symbol RBM15B
Gene ID (NCBI) 29890
RRIDAB_2882729
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8NDT2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).

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