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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RBM11 Polyclonal antibody

RBM11 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 17220-1-AP

Synonyms

RBM11, RNA binding motif protein 11

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 17220-1-AP

    WB analysis of HEK-293 using 17220-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17220-1-AP (RBM11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 17220-1-AP

    WB analysis of HEK-293 using 17220-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17220-1-AP (RBM11 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IP experiment of HEK-293 using 17220-1-AP

    IP experiment of HEK-293 using 17220-1-AP

    IP result of anti-RBM11 (IP:17220-1-AP, 4ug; Detection:17220-1-AP 1:300) with HEK-293 cells lysate 3000ug.

  • IF Staining of HEK-293 using 17220-1-AP

    IF Staining of HEK-293 using 17220-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 17220-1-AP (RBM11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17220-1-AP targets RBM11 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM11 fusion protein Ag11040 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 11
Calculated molecular weight 281 aa, 32 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC030196
Gene symbol RBM11
Gene ID (NCBI) 54033
RRIDAB_2175729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RBM11 antibody 17220-1-APDownload protocol
IF protocol for RBM11 antibody 17220-1-APDownload protocol
IP protocol for RBM11 antibody 17220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dis Markers

RNA-Binding Motif Protein 11 (RBM11) Serves as a Prognostic Biomarker and Promotes Ovarian Cancer Progression.

Authors - Chunhong Fu
  • KD Validated
View Article